Development of PCR assay based on ITS2 rDNA polymorphism for the detection and differentiation ofFusarium sporotrichioides

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Development of PCR assay based on ITS2 rDNA polymorphism for the detection and differentiation of Fusarium sporotrichioides.

A polymerase chain reaction assay was developed for detection of Fusarium sporotrichioides, a plant pathogen in many parts of the world. Based on small nucleotide differences in ITS2 (Internal Transcribed Spacer) rDNA of our local isolate of F. sporotrichioides (Accession No. AY510069) and other isolates found in NCBI/GeneBank database, species specific primer FspITS2K was selected. Primer pair...

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A Consistent PCR-RFLP Assay Based on ITS-2 Ribosomal DNA for Differentiation of Fasciola Species

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ژورنال

عنوان ژورنال: FEMS Microbiology Letters

سال: 2004

ISSN: 0378-1097,1574-6968

DOI: 10.1016/j.femsle.2004.08.037